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You Searched For: Protein Extraction

Protein extraction kits provide a simple, rapid, and reproducible method for preparing total cellular protein extracts from a wide variety of biological samples. The results of these extraction systems generate protein samples that can be applied directly to a variety of applications. High-performance models are designed for efficient recovery of genomic DNA and RNA. These extraction kits are capable of purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids.

Protein extraction kits provide a simple, rapid, and reproducible method for preparing total cellular protein extracts from a wide variety of biological samples. The results of these extraction systems generate protein samples that can be applied directly to a variety of applications. High-performance models are designed for efficient recovery of genomic DNA and RNA. These extraction kits are capable of purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids.


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ProteoExtract® Albumin/IgG Removal Kit, Maxi

Supplier: Merck Millipore (Calbiochem‎)
Description: ProteoExtract® Kits cover the different steps of the proteomics workflow, from protein extraction and abundant protein removal to concentration of protein mixtures and removal of interfering substances, digestion of proteins and selective capturing of phosphopeptides. These kits work in concert with one another to provide protein samples that are directly compatible with downstream analytical applications.

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Endoproteinase Asp-N, excision grade, Pseudomonas fragi

Catalog Number: (324708-2)
Supplier: Merck Millipore (Calbiochem‎)
Description: Native endoproteinase Asp-N from <i>Pseudomonas fragi.</i> Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyses proteins at the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
UOM: 1 * 2 µG
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Endoproteinase Glu-C, excision grade, Staphylococcus aureus

Catalog Number: (324713-50)
Supplier: Merck Millipore (Calbiochem‎)
Description: Native endoproteinase Glu-C from <i>Staphylococcus aureus</i>. Serine protease that specifically hydrolyses peptide bonds at the carboxylic side of glutamic acid and aspartic acid residues. Inhibited by DFP and α2-macroglobulin. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
UOM: 1 * 50 µG
Product Image-PIER90305

Trypsin Protease, 5×20 µg

Supplier: Thermo Fisher Scientific
Description: Pierceâ„¢ Trypsin Protease, MS Grade, is highly purified porcine trypsin that has been chemically modified for maximum activity and stability in proteomic applications.

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Endoproteinase Lys-C, sequencing grade, Lysobacter enzymogenes

Catalog Number: (324715-5)
Supplier: Merck Millipore (Calbiochem‎)
Description: Native endoproteinase Lys-C from <i>Lysobacter enzymogenes</i>. Serine protease that specifically hydrolyses amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease: protein by weight) for sequence analysis.
UOM: 1 * 5 µG
Product Image-PIER90054

Endoproteinase Glu-C protease, MS grade, Pierceâ„¢

Catalog Number: (PIER90054)
Supplier: Thermo Fisher Scientific
Description: Glu-C protease is a highly specific endoproteinase use to improve sequence coverage in mass spectrometry protein identification applications.
UOM: 1 * 50 µG

MSDS


Product Image-PIER90056

Endoproteinase chymotrypsin protease (TLCK treated), MS grade, Pierceâ„¢

Catalog Number: (PIER90056)
Supplier: Thermo Fisher Scientific
Description: Chymotrypsin protease is a purified native endoproteinase validated for maximum activity and selectivity in proteomic applications.
UOM: 1 * 100 µG
Product Image-PIER90053

Endoproteinase Asp-N protease, MS grade, Pierceâ„¢

Catalog Number: (PIER90053)
Supplier: Thermo Fisher Scientific
Description: Asp-N protease is a highly specific endoproteinase used to improve sequence coverage in mass spectrometry protein identification applications.
UOM: 1 * 2 µG

MSDS


Product Image-786-599

Iminobiotin resin, immobilised

Catalog Number: (786-599)
Supplier: G-Biosciences
Description: Immobilised Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose. The resin allows for the purification of avidin, streptavidin and Neutravidin and their subsequent gentle elution using non denaturing elution buffers.
UOM: 1 * 5 mL
Product Image-786-680

ProteinOUTâ„¢ washing solution

Catalog Number: (786-680)
Supplier: G-Biosciences
Description: Protein-OUTâ„¢ is a washing solution that cleans plastic and glassware used in the proteolytic digestion and peptide isolation for mass spectrometry analysis. The rinsing of plastic and glassware with Protein-OUTâ„¢ will remove interfering chemicals and peptides that can leach from the tube walls and interfere or suppress the mass spectrometry signal. In addition, rinsing with Protein-OUTâ„¢ removes inadvertent contamination with dust and keratin.
UOM: 1 * 50 mL
Product Image-786-791

Pepsin, immobilised

Catalog Number: (786-791)
Supplier: G-Biosciences
Description: A proteolytic enzyme immobilised on 4% agarose that is routinely used for the generation of F(ab’)₂ fragments from Immunoglobulin G (IgG). The pepsin has the ability to cleave the heavy chains near the hinge region. The immobilised Pepsin offers the distinct advantage of eliminating enzyme contamination of the F(ab)₂ fragments.
UOM: 1 * 1 items
Product Image-786-790

Papain, immobilised

Catalog Number: (786-790)
Supplier: G-Biosciences
Description: A cysteine protease enzyme (EC 3.4.22.2) immobilised on 4% agarose, cleaves Immunoglobulin G (IgG) antibody molecules in the hinge region, generating three ~50 kDa fragments; two Fab domains and a Fc domain. The papain-digested antibody is unable to promote agglutination, precipitation, opsonisation, and lysis.
UOM: 1 * 1 items
Product Image-786-252

AlbuminOUTâ„¢

Supplier: G-Biosciences
Description: Samples that contain a large abundance of albumin, such as plasma and cerebrospinal fluid, tend to mask identification and discovery of other less abundant proteins in two dimensional gel electrophoresis and other studies. AlbuminOUTâ„¢ has been specifically developed for substantial removal of albumin from such samples.

Product Image-PIER88216

Micrococcal Nuclease Solution (≥100 U/µL), 150 µl

Catalog Number: (PIER88216)
Supplier: Thermo Fisher Scientific
Description: This Micrococcal Nuclease (MNase) is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.
UOM: 1 * 1 items
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Imjectâ„¢ Ovalbumin, malemide-activated

Catalog Number: (PIEA77126)
Supplier: Thermo Fisher Scientific
Description: Imjectâ„¢ Maleimide-activated Ovalbumin is sulfhydryl-reactive ovalbumin (OVA) for hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens.
UOM: 1 * 2 mg

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Kit with 100 ml BugBuster® Protein Extraction Reagent and 200 µl Lysonase™ Bioprocessing Reagent

Supplier: Merck Millipore (Novagen)
Description: BugBuster® Plus Lysonase™ includes BugBuster Protein Extraction Reagent and Lysonase Bioprocessing Reagent, an optimised, ready-to-use blend of rLysozyme™ and Benzonase® Nuclease in a single kit. This powerful combination of two reagents allows maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The BugBuster® and rLysozyme™ efficiently lyse the bacterial cells while the Benzonase® degrades nucleic acids to facilitate downstream processing. Requires 5 ml BugBuster and 10 µl Lysonase per 1 g cell paste. The kits provide sufficient reagents for Protein Extraction from 20 g or 100 g cell paste.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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