Luminaris Color HiGreen qPCR Master Mixes are universal ready to use solutions optimised for qPCR and two-step RT-qPCR. The master mixes include hot start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimised PCR buffer. Only the template and primers need to be added. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.
- UDG provides built-in carry-over contamination control
- Colour change system to track pipetting
- Versions with pre-mixed ROX or fluorescein for different real time platforms
The Hot Start Taq DNA polymerase in combination with an optimised buffer ensures PCR specificity and sensitivity. dUTP and UDG are included in the mix for carry-over contamination control. The Luminaris Color HiGreen qPCR Master Mixes are supplemented with an inert blue dye and a separate yellow sample Buffer that contains a yellow dye. The qPCR reaction mix containing both components is green.
The use of Luminaris Color HiGreen qPCR Master Mixes in qPCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. Individual formulations of Luminaris Color HiGreen qPCR Master Mixes with ROX at different concentrations or fluorescein as passive reference dyes are available for most qPCR platforms.