Protein extraction kit, Yeast PE LB™

Supplier: G-Biosciences
786-178EA 283 EUR
786-178 786-179
Protein extraction kit, Yeast PE LB™
Buffers Extraction Buffers
Yeast PE LB™ kit has been developed for extraction of biologically active, soluble proteins from yeast cells. Yeast PE LB™ is a proprietary improvement on the lyticase (Zymolyase®) based spheroplast preparation and extraction of soluble proteins from yeast cells. Yeast PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins.

  • Eliminates the need for glass bead lysis
  • Supplied as a kit containing Zymolyase® and reagents needed for protein extraction
  • Suitable for processing 100×50 µl yeast cell pellet preparations
  • Suitable for most applications including enzyme and protein purification applications, electrophoresis, Western blotting and 2D-gel analysis

A ready to use Zymolyase® preparation is also provided. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Yeast PE LB™. Yeast PE LB™ has been tested on several widely used yeast strains.

The proprietary combination of this reagent provides a simple and versatile method of yeast protein extraction. Yeast PE LB™ eliminates the need for laborious glass bead lysis of yeast cells.

Delivery information: Yeast PE LB™ buffer is also available separately for further downstream processing.
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