Samples that contain a large abundance of albumin, such as plasma and cerebrospinal fluid, tend to mask identification and discovery of other less abundant proteins in two dimensional gel electrophoresis and other studies. AlbuminOUT™ has been specifically developed for substantial removal of albumin from such samples.
The albumin removal method is based on the binding of albumin with Cibacron Blue dye. AlbuminOUT™ has been optimised for removal of human albumin from samples. AlbuminOUT™ uses a rapid spin column method, where each column contains 0,2 ml dye-bound resins with capacity of greater than 2 mg human albumin per column. AlbuminOUT™ will remove over 98% albumin from 5 to 50 μl human plasma. The spin column format allows removal of albumin within ten minutes.
The high capacity blue dye-binding resin allows instantaneous binding and removal of albumin from human, pig, sheep, dog, rabbit, rat, and bovine samples. AlbuminOUT™ may also be used for removal of albumin from other species.