Thrombin is a protease used to digest fusion proteins prepared from pGEX vectors containing the recognition sequence for thrombin (pGEX-1lT, pGEX-2T, pGEX-2TK, pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3).
- Enables the site-specific cleavage of fusion proteins containing an accessible thrombin recognition sequence
- Purified from bovine plasma
- Functionally free of other clotting factors, plasminogen, and plasmin
- Can be used either following affinity purification or while fusion proteins are bound to Glutathione Sepharose™ 4B, Glutathione Sepharose™ High Performance, or Glutathione Sepharose™ 4 Fast Flow
Cleavage Buffer: PBS 140 mM NaCl, 2,7 mM KCl, 10 mM Na₂HPO₄, 1,8 mM KH₂PO₄, pH 7,3
Elution buffer: 50 mM Tris-HCl, 10 mM reduced glutathione, pH 8,0.
Inhibitors: Pefabloc TH benzamidine, AEBSF, APMSF, antithrombin III, Antipain, α1-antitrypsin, aprotinin, chymostatin, hirudin, leupeptin, PMSF.
Molecular weight: 37000.
Unit definition: One unit of enzyme cleaves 90% of 100 μg of a test GST fusion protein when incubated in 1X PBS at 22 °C for 16 hours.
Ordering information: Supplied as lyophilised powder.