Lysine-C™, sequencing grade

Supplier: G-Biosciences
786-14EA 695 EUR
Lysine-C™, sequencing grade
Nucleic Acid Reagents Sanger Sequencing Reagents
SG-Lysine-C™ endopeptidase, from Lysobacter enzymogenes, is a serine protease highly specific in cleaving peptide bonds at the carboxy side of lysine. Highly purified preparations of SG-Lysine-C™ are chemically modified making the enzyme resistant to autolysis and stabilising its enzymatic activity.

  • Modified lysine-C for sequence analysis
  • Resistant to autolysis and degradation
  • High specificity, free from other known endopeptidases
  • Consistency of activity levels from lot to lot for reproducible digestions

SG-Lysine-C™ is supplied lyophilised in 5 μg vials. The enzyme is typically reconstituted to a concentration of 0,25 μg/ml. For fragmentation, the enzyme is added to the sample protein in a ratio of 1:100 to 1:20 (enzyme to protein, by weight) in a standard digestion buffer.
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