NICK™ columns were originally designed for purification of nick-translated DNA fragments but are also suitable for separation of any labelled probe from unincorporated labelled nucleotides.
- For rapid purification of labeled DNA (20 bases in length) from unincorporated radiolabeled nucleotides using gravity-flow chromatography
- Prepacked with Sephadex™ G-50 DNA Grade in distilled water with 0,15% Kathon CG/ICP Biocide
- Can accommodate sample volumes up to 100 μl
They are ready for immediate use, and operate by gravity flow. Tests show that at least 97% of radioactivity applied in a nick-translated mixture elutes in two well-separated peaks, corresponding to labeled DNA and unincorporated nucleotides, respectively. At least 90% of the applied DNA is recovered.