Affinity chromatography kit, His GraviTrap™

Supplier: Cytiva
28-4013-51EA 530 EUR
Affinity chromatography kit, His GraviTrap™
Chromatography Columns
His GraviTrap™ is a prepacked, single-use column for purification of histidine-tagged proteins by immobilised metal affinity chromatography (IMAC). The column gives fast and simple gravity-flow purifications without any need for a purification system. Large sample volumes can be applied in one go, and the histidine-tagged protein is effectively eluted in a small volume.

  • Short purification times, ~30 minutes
  • High protein binding capacity, ~40 mg/column
  • Purifies unclarified samples

His GraviTrap contains Ni Sepharose™ 6 Fast Flow medium, which has negligible nickel leakage and is compatible with denaturing and reducing agents as well as a wide range of additives. The columns are made of biocompatible polypropylene. Special frits protect the medium from running dry during purification.

Delivery information: Each kit contains two packs of His GraviTrap™ and one pack of His buffer kit, sufficient for 20 preps.
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