His SpinTrap™ TALON® is a single-use column designed for high purity, small-scale purification and screening of histidine-tagged proteins from clarified cell lysates.
- Suitable for purification of multiple samples in parallel, such as screening
- With a microcentrifuge, one run takes about 10 minutes
Convenient, ready-to-use single-use spin column, prepacked with 100 μl TALON® Superflow™ cobalt-IMAC (immobilized metal affinity chromatography) medium offering a different selectivity compared to nickel-charged media.
The columns make parallel preparations of few or many small-scale cell lysates quick and easy. Prepacked columns enable highly reproducible column to column purification and yield, with RSD <6%. Leakage of cobalt ions from TALON® Superflow™ medium is negligible.
His SpinTrap TALON® columns are used together with a standard microcentrifuge and purification takes approximately 10 minutes.