ECL protein biotinylation module for labelling of antibodies or other proteins in solution; for use in western blotting and immunoprecipitation experiments; for labelling of cell surface molecules and total protein detection on blots.
- A complete system for protein biotinylation and detection using streptavidin-HRP and ECL reagents
- Detects less than 5 pg solution-labelled mouse IgG and less than 1 ng membrane-labeled transferrin
Delivery information: Each system contains the following reagents, sufficient for either 5×2,5 mg solution labellings, or twenty-five 8×10 cm membrane labellings: biotinylation reagent, sodium bicarbonate buffer, five Sephadex™ G-25 columns, streptavidin-HRP conjugate, blocking reagent, ECL western blotting detection reagents 1 and 2 (125 ml each).