GST Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of GST-tagged proteins according to recommended protocols.
- Premade stock solutions of buffers optimized for GST-tagged protein purification
- Sufficient reagents are supplied to purify up to 20 mg of GST-tagged protein
Can be used in combination with a number of column types, such as GST SpinTrap™, GST MultiTrap™ FF, GST MultiTrap 4B, GST GraviTrap™, GSTrap™ FF, GSTrap™ 4B and GSTrap™ HP.
Delivery information: Includes 50 ml 10 × PBS stock solution pH 7.3 (1.4 M NaCl, 27 mM KCl, 101 mM Na2HPO4, 18 mM KH2PO4, pH 7.4); Reduced Glutathione 0.154 g. Addition of 50 ml of glutathione dilution buffer results in a solution of 10 mM glutathione, 50 mM Tris-HCl (pH 8.0); 50 ml dilution buffer: 50 mM Tris-HCl (pH 8.0).