DNA modification enzymes

Supplier: Cytiva
E70092YEA 105.82 EUR
E70092Y E70092Z E70092X
DNA modification enzymes
Enzymes
A high specific activity, heat-labile alkaline phosphatase. Completely and irreversibly inactivated in Tris buffers at pH 8,0 to 8,5 by heating for 15 minutes at 65 °C. No further treatment is necessary.

  • Removes 5'-phosphates from DNA and RNA
  • Easily inactivated by heat

Alkaline phosphatase is suitable for de phosphorylation of 5' and 3'-overhangs, recessed ends, blunt ends, and single nucleotides. Following incubation, illustra Alkaline Phosphatase can be easily inactivated by heating.

Use together with illustra Exonuclease I for cleaning up PCR reactions prior to downstream application such as DNA sequencing.

Unit Definition: One unit is the amount of the enzyme required to dephosphorylate 1 Â µg of linearized pUC57 DNA termini in 10 minutes at 37 °C.

Delivery information: Includes 10X reaction buffer: 200 mM Tris-HCl, pH 8,0, 100 mM MgCl₂; dilution buffer: 50 mM Tris-HCl, pH 8,0.
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