Cell culture salts, salt solutions and buffers

Supplier: Biowest
P2035-KGEA 19.5 EUR
P2035-KG P0750-N10L L0612-500 P0750-N1L P2060-KG P0750-N50L L0640-500 L0615-500 L0606-500 P0153-N1L P0153-N10L L0615-100 L0611-500 L0601-500 X0513-500 L0680-100 L0680-500 X0509-500 P2064-N5L P5455-KG L0180-500 L0615-1000 X0520-500 P2060-500GR L0642-100 P0154-N10L P2066-KG L0180-100 X0515-100 L0643-500 X0515-500 L0606-100 P2035-500GR X0507-500 L0605-500 L0615-C10LS P5455-100GR P0154-N50L P0154-N1L L0642-500 X0112-500 P0750-N5L X0112-100 X0510-500 L0643-100 L0607-500 L0611-100 X0113-500
Cell culture salts, salt solutions and buffers
Buffers Cell Culture Buffers
These irrigating buffers and salt solutions are sterile, physiological balanced solutions intended for use in the maintenance of mammalian cells, where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. Applications include the intermediate steps for cultivation of cells, like washing, centrifugation, suspending and counting, as well as for many analytical methods or biochemical treatments. Principally these solutions should be used to keep the cells in suspension only for minutes or a few hours maximum. These buffers and solutions are not cell culture media.

They are comprised of a phosphate buffer system, sodium chloride to adjust the osmolarity and perhaps sugar for short term nutrition and stabilisation of morphology. For applications where Ca²⁺ and Mg²⁺ ions interfere with enzyme activity (eg. Trypsin) the modified buffers without calcium and magnesium should be used.
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