The proprietary hydrophilic bonded layer in these MAbPac SEC-1 Columns results in minimal non-desired interactions between the proteins and the stationary phase. The stationary phase is designed to handle different eluent conditions containing both high and low ionic strength mobile phases, as well as mass spectrometry friendly volatile eluents.
- Hydrophilic bonded layer minimises undesired interactions between the biomolecules and the stationary phase
- Non metallic and biocompatible PEEK housing eliminates metal contamination from the column hardware
The Thermo Scientific™ MAbPac™ SEC-1 is a size exclusion chromatography (SEC) column designed for mAb characterisation, including the separation of monomers, aggregates, and fragments. The unique chemistry is stable under both denaturing and non-denaturing conditions, using high-salt, low-salt, or volatile mobile phases.
The MAbPac SEC-1 column is based on high-purity, spherical, porous (300 Å), 5 μm silica particles covalently modified with a proprietary diol hydrophilic layer. The column is packed into three different int.Ø formats (7,8, 4,0, and 2,1 mm) to accommodate different applications and sample loadings. The 7,8 mm int.Ø column provides the highest resolution of mAb and aggregates separation and rugged method for routine analysis. The 4,0 mm int.Ø column offers excellent resolution at greatly reduced mobile phase consumption and requires modern HPLC system with minimal extra column volumes. The 2,1 mm int.Ø column provides the highest sensitivity for small amount of sample loading, and is suitable for direct MS detection.
Columns feature rugged, reproducible packing. Stable surface bonding leads to low column bleed and compatibility with MS, ELSD and Thermo Scientific™ Dionex™ Corona™ Aerosol Detection. These columns provide superior performance for the analysis of monoclonal antibodies, aggregates, and their fragments.