GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.
GenLysate™ total protein lysates are ready to use for SDS-PAGE and Western blotting purposes. The total proteins are being extracted from whole tissue or cells following the method of Laemmli. The proteins are extracted in a denaturing buffer containing 62 mM Tris HCl and 2% SDS with ProteaseARREST™ protease inhibitor cocktail. This minimises proteolytic damage to the proteins.
GenLysate™ total protein lysates are offered as a 150 µg lyophilised protein per vial and, following reconstitution in DI water, are ready for use. GenLysate™ is also available in a wide variety of other species and tissues. Species include: Human (both normal tissue and tumour tissue lysates available), mouse, rat, rabbit, pig, plant-related, bacteria, insect, bird, frog, fish, marsupial, earthworm, and yeast.