Cross-linking agents are divided into groups dependent on the number and similarity of the reactive groups. Homobifunctional cross-linkers are used in one step reactions while the heterobifunctional cross-linkers are used in two-step sequential reactions, where the least labile reactive end is reacted first. Homobifunctional cross-linking agents have the tendency to result in self-conjugation, polymerization, and intracellular cross-linking. On the other hand, heterobifunctional agents allow more controlled two step reactions, which minimizes undesirable intramolecular cross-reaction and polymerisation.
Cross-linking agents are used for the following applications: Protein structural and functional studies, cell membrane structural studies, protein and receptor interactions, cell surface studies, solid-phase immobilisation, preparation of immunogens, immunotoxins and other conjugated protein reagents, protein to DNA/RNA cross-linking, reactive group transfer, protein-protein conjugation, and for immunological tools.
Delivery information: Double Do™ cross-linkers include the chemical cross-linker, supporting accessories consisting of specific reaction buffers, complete reactions systems, selection guides and protocols.