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Anti-p14ARF Rabbit Polyclonal Antibody

Supplier: Thermo Scientific
Suggested positive control: BT549 or HeLa whole cell extracts, antigen standard for CDKN2A (transient overexpression lysate). The INK4a-ARF locus is comprised of two tumor suppressors, p16INK4a and p14ARF. These two proteins are encoded through differential splicing of alternative first exons. The p16INK4a (exon 1alpha) protein inhibits the cyclin D-dependent kinases (CDK) that control the phosphorylation of the Rb protein and cell proliferation. The p14ARF gene product complexes with the MDM2 protein within the nucleus, thus modulating the activity of the p53 protein. P14ARF is a potent tumor suppressor in the presence of wild-type p53, while mutant p53 substantially reduces growth inhibition by p14ARF.

Type: Primary
Antigen: p14ARF
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: IgG
Reactivity: Human



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Size Pk VWR Catalog Number Quantity
100 µg 100 µl PIEAPA116661 Blocked
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SPECIFICATIONS

Antigen symbol p14ARF
Antigen name Cyclin-dependent kinase inhibitor 2A
Reactivity Human
Conjugation Unconjugated
Clonality Polyclonal
Antibody type Primary
Host Rabbit
Isotype IgG
Western Blot Yes
ELISA Yes
ImmunoChemistry Yes
Antigen synonyms CDKN2A, Cell cycle negative regulator beta, CDK4 inhibitor p16INK4, CDK4I, ARF, Cyclin dependent kinase inhibitor 2A, CDKN2, CMM2, Cyclin dependent ki
Gene ID 1029
Concentration 1 mg/ml
Immunogen A synthetic peptide to human p14ARF (internal sequence)
Purification Antigen affinity chromatography
Form Liquid
Storage Buffer tris citrate, 0.1% sodium azide, pH 7-8
Storage temperature 4° C, do not freeze
Shipping Temperature Ice
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