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Anti-NEFM Chicken Polyclonal Antibody

Supplier: Thermo Scientific
Suggested positive control: antigen standard for NEFM (transient overexpression lysate). Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. In the electron microscope neurofilaments appears as 10nm diameter fibres of indeterminate length which generally have fine wispy protrusions from their sides. They are found particularly abundantly in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid conduction of impulses down axons. They are composed a mixture of subunits which usually include the three neurofilament triplet proteins, known as NF-L, NF-M and NF-H. Neurofilaments may also include smaller amounts of peripherin, a-internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable.

Type: Primary
Antigen: NEFM
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Chicken
Isotype: IgY
Reactivity: Mouse, Chicken, Feline, Fish, Human, Rat



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Size Pk VWR Catalog Number Quantity
250 µL 250 µl PIEAPA116758 Blocked
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SPECIFICATIONS

Antigen symbol NEFM
Antigen name Neurofilament, Medium chain
Reactivity Human, Fish, Feline, Mouse, Rat, Chicken
Conjugation Unconjugated
Clonality Polyclonal
Antibody type Primary
Host Chicken
Isotype IgY
Western Blot Yes
ImmunoFluorescence Yes
Antigen synonyms neurofilament medium polypeptide, neurofilament 3, NEF3, NFM, NF-M, NMC,160 kD neurofilament protein
Gene ID 396206, 4741, 24588, 101081291, 18040
Immunogen The C-terminal extension of rat NF-M (KE segment)
Purification Ammonium sulfate precipitation
Form Liquid
Storage Buffer PBS, 10mM sodium azide, pH 7.2
Storage temperature Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping Temperature Ice
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