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Anti-CENPF Rabbit Polyclonal Antibody

Supplier: Thermo Scientific

Suggested positive control: HeLa and SK-N-SH cells. The kinetophore is a macromolecular structure that is associated with the centromeres of chromosomes and is responsible for establishing and maintaining the connections with the microtubules of the mitotic spindle. The proteins that reside within the chromosomal domain fall into two broad classes: those that associate with the centromere throughout the cell cycle and those that are found at the centromere in a cell cycle specific manner. Members of the first class include CENP-A, CENP-B, and CENP-C. The members of the second class include several ubiquitous cellular proteins such as calmodulin, tubulin, dynein and p34 cdc2 and also CENP-E and CENP-F. CENP-F appears to be the earliest member to interact with the centromere-kinetochore complex. CENP-F also shows a cell-cycle distribution that is both temporally regulated and diverse in terms of the structural components it localizes to and therefore may play a role in several mitotic events.

Type: Primary
Antigen: CENPF
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: IgG
Reactivity: Human, Bovine



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Size Pk VWR Catalog Number Quantity
50 µL 50 µl PIEAPA116826 Blocked
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SPECIFICATIONS

Antigen symbol CENPF
Antigen name Centromere Protein F, 350/400kDa
Reactivity Bovine, Human
Conjugation Unconjugated
Clonality Polyclonal
Antibody type Primary
Host Rabbit
Isotype IgG
Western Blot Yes
ImmunoPrecipitation Yes
ImmunoFluorescence Yes
Flow Cytometry Yes
Antigen synonyms Centromere Protein F, CENF, Mitosin, CENP F Kinetochore Protein, AH Antigen, CENPF
Gene ID 1063
Concentration 2.5 mg/ml
Immunogen A bacterial fusion protein from the C-terminus of CENP-F.
Purification Affinity chromatography
Form Liquid
Storage Buffer PBS, 50% glycerol, 0.05% sodium azide
Storage temperature -20° C, Avoid Freeze/Thaw Cycles
Shipping Temperature Ice
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