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Anti-LC3 Rabbit Polyclonal Antibody

Supplier: Thermo Scientific
The reaction with mouse LC3 is weaker than human. The immunogen for this antibody production has 100% sequence homology with rat and zebrafish, 92% with mouse, and 91% with bovine proteins. LC3, a mammalian homologue of Apg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. However, LC3 is now thought to also be involved in autophagy. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. LC3-II is the first mammalian protein identified that specifically associates with the autophagosome membranes.

Type: Primary
Antigen: LC3
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: IgG
Reactivity: Hamster, Primates, Canine, Bacteria, Bovine, Human, Rat, Porcine, Mouse, Zebrafish



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Size Pk VWR Catalog Number Quantity
100 µL 100 µl PIEAPA116930 Blocked
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SPECIFICATIONS

Antigen symbol LC3
Antigen name 1A/1B-light chain 3
Reactivity Rat, Human, Dog, Mouse, Zebrafish, Bacteria, Hamster, Bovine, Primates, Porcine
Conjugation Unconjugated
Clonality Polyclonal
Antibody type Primary
Host Rabbit
Isotype IgG
Western Blot Yes
ELISA Yes
ImmunoChemistry Yes
ImmunoPrecipitation Yes
ImmunoFluorescence Yes
Antigen synonyms MAP1 light chain 3-like protein 2 a, Microtubule-associated proteins 1A/1B light chain 3B MAP1A/MAP1B LC3 B antibody, MAP1A/b light chain 3 B antibody
Gene ID 81631, 322425, 408001, 67443, 64862, 100462754
Concentration 1 mg/ml
Immunogen Synthetic peptide between amino acids 1-100 of the human LC3 protein.
Purification Antigen affinity chromatography
Form Liquid
Storage Buffer PBS, 0.02% sodium azide
Storage temperature -20° C, Avoid Freeze/Thaw Cycles
Shipping Temperature Ice
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