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Anti-H3 Rabbit Polyclonal Antibody

Supplier: Thermo Scientific

This antibody is expected to cross react with all mammals and a wide range of other species based on sequence homology. Suggested positive control: Hela whole cell lysate, MEF and HeLa lysates, MEF whole cell lysate0. In diploid eukaryotic cells, the chromatin fibers are about 20nM in diameter. They consist of two major components in equal amounts, DNA and basic proteins called histones. Histones are believed to be regularly arranged in the deep grove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers.

Type: Primary
Antigen: H3
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: IgG
Reactivity: Mouse, Human



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Size Pk VWR Catalog Number Quantity
100 µL 100 µl PIEAPA116941 Blocked
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SPECIFICATIONS

Antigen symbol H3
Antigen name Histone H3
Reactivity Human, Mouse
Conjugation Unconjugated
Clonality Polyclonal
Antibody type Primary
Host Rabbit
Isotype IgG
Western Blot Yes
Antigen synonyms H3a, HIST1H3A, histone H3/a, Histone 1, Histone H3, Histone H3.1
Gene ID 8350, 360198
Concentration 18 mg/ml
Immunogen Synthetic peptide between amino acids 100-135 of the human Histone H3 protein.
Purification Ammonium sulfate precipitation
Form Liquid
Storage Buffer PBS, 0.02% sodium azide
Storage temperature -20° C, Avoid Freeze/Thaw Cycles
Shipping Temperature Ice
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