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Anti-VEGFA Rabbit Polyclonal Antibody

Supplier: Thermo Scientific

In Western blot, a band is seen at ~42 kDa with a chimera transfected lysate representing the homodimer VEGFA. Another faint band is seen at ~21 kDa, representing the monodomer form of VEGFA. This antibody is not recommended for IHC applications.Suggested positive control: antigen standard for VEGFA (transient overexpression lysate). VEGFA is a member of the PDGF/VEGF growth factor family and is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. VEGF and its high-affinity binding receptors, the tyrosine kinases FLK1 and FLT1, are thought to be important for the development of embryonic vasculature. It has been shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity, playing an inhibitory role in angiogenesis.

Type: Primary
Antigen: VEGFA
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: IgG
Reactivity: Rat, Bovine, Chicken, Equine, Human, Guinea Pig, Canine, Mouse



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Size Pk VWR Catalog Number Quantity
100 µL 100 µl PIEAPA116948 Blocked
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SPECIFICATIONS

Antigen symbol VEGFA
Antigen name Vascular endothelial growth factor A
Reactivity Mouse, Guinea Pig, Chicken, Bovine, Equine, Dog, Human, Rat
Conjugation Unconjugated
Clonality Polyclonal
Antibody type Primary
Host Rabbit
Isotype IgG
Western Blot Yes
ELISA Yes
Antigen synonyms Vascular permeability factor antibody, Vascular endothelial growth factor A precursor antibody, Vascular endothelial growth factor A antibody, VPF ant
Gene ID 7422, 100033839, 100462897, 83785, 281572, 22339, 403802, 395909
Concentration 1 mg/ml
Immunogen Synthetic peptide between amino acids 150-250 of the internal region of human VEGFA protein sequence.
Purification Antigen affinity chromatography
Form Liquid
Storage Buffer tris phosphate, tris citrate, 0.09% sodium azide, pH 7-8
Storage temperature 4° C, do not freeze
Shipping Temperature Ice
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