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Donkey Anti-Human IgG (H+L) Polyclonal Antibody (Biotin)

Supplier: Rockland Immunochemical

This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid)) as a substrate for 30 minutes at room temperature.

Recommended Dilutions: ELISA: 1:20000 - 1:100000; Western Blot: 1:2000 - 1:10000; Immunochemistry: 1:1000 - 1:5000;
Restore with deionized water (or equivalent); 1.0 ml

Type: Secondary
Antigen: IgG
Clonality: Polyclonal
Clone:
Conjugation: Biotin
Epitope: Heavy and Light chain
Host: Donkey
Isotype:
Reactivity: Human



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Size Pk VWR Catalog Number Quantity
2 mg 2 mg ROCK609-706-002
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SPECIFICATIONS

Antigen symbol IgG (H+L)
Antigen name IgG (H+L)
Reactivity Human
Conjugation Biotin
Clonality Polyclonal
Antibody type Secondary
Host Donkey
Isotype none
Western Blot Yes
ELISA Yes
ImmunoChemistry Yes
Concentration 2,0 mg/ml by UV absorbance at 280 nm
Format Antibody fraction
Cross Adsorption non
Immunogen Human IgG whole molecule
Purification purified by immunoaffinity chromatography
Form Lyophilized
Storage Buffer 0,02 M Potassium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 0,01% (w/v) Sodium Azide, 10 mg/ml Bovine Serum Albumin, Immunoglobulin and Protease free
Storage temperature Cool
Shipping Temperature Ice
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