In this experiment, students gain experience in non-isotopic DNA detection and the use of Southern Blot analysis in DNA fingerprinting for a hypothetical paternity test. Includes three modules: Agarose gel electrophoresis, Southern blot transfer, and non-isotopic detection of DNA.
- For 5 groups
- Electrophoresis 45 minutes, blotting overnight, non-isotopic detection 3 - 4 hours
Also required: Electrophoresis apparatus & power supply, automatic micro-pipette with tips, balance, microwave or hot plate, waterbath, incubation oven, pipette pumps or bulbs, pipettes, floating racks for microtest tubes, laboratory glassware, plastic wrap, distilled or deionised water, NaCl, NaOH, concentrated HCl, ice. Note: Some components require freezer or refrigerator storage.
Delivery information: Kit includes instructions, predigested DNA samples, buffers, NBT/BCIP tablets, streptavidin-alkaline phosphatase, nylon
membranes, filter paper, UltraSpec-Agarose™ powder.