HisPur™ Ni-NTA superflow agarose, Pierce™

Supplier: Thermo Fisher Scientific
HisPur™
PIER25215EA 738 EUR
PIER25215 PIER25214 PIER25217 PIER25216
HisPur™ Ni-NTA superflow agarose, Pierce™
Protein Purification Protein Purification Resins
Pierce™ HisPur™ Ni-NTA Superflow Agarose is a nitrilotriacetic acid modified Superflow 6 support charged with divalent nickel (Ni⁺²) used for FPLC purification of poly-histidine-tagged proteins.

  • Binds greater than 20 mg of His-tagged GFP per ml of resin at a linear flow rate of 300 cm/hour
  • Provides greater than 80% purity of eluted fractions when used to purify from lysates
  • Can be used to purify proteins under native and denaturing conditions
  • Highly crosslinked beads tolerate linear flow rates up to 1200 cm/hour
  • Maintains function in a wide variety of chemicals and pH conditions
  • Resin is stable through multiple cycles of cleaning and reuse

HisPur™ Ni-NTA Superflow Agarose is a durable resin that does not compress at flow rates used for medium- or large-scale FPLC purifications.
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