UltraLink™ Biosupport is a durable, azlactone-activated, beaded-polyacrylamide resin to covalently immobilise proteins and other primary amine biomolecules for preparation of affinity purification columns.
- With excellent reusability
- Immobilises proteins with very high efficiency and coupling capacity in 1 hour
- Porous beads have a 60 µm diameter, can withstand 6,9 bar and allow for linear flow-through rates of 3000 cm/hour
UltraLink™ Biosupport is an alternative to CNBr- and NHS-activated agarose resins for immobilising antibodies and other proteins to prepare affinity columns. The beaded resin is a hydrophilic copolymer of polyacrylamide and azlactone having a rigid polymeric structure with high surface area and pore volume. The azlactone groups react rapidly and efficiently with primary amines (eg. side chain of lysine residues) to covalently immobilise protein or other amine-containing ligands. The bead structure and efficient coupling chemistry of UltraLink™ Biosupport results in high protein binding capacity, high linear flow rates, low non specific binding and overall superior performance in affinity chromatography. UltraLink™ resins are ideal for medium pressure applications such as FPLC.