UltraLink™ Monomeric Avidin resin is ideal for affinity purification of biotinylated proteins, peptides and other molecules. Immobilisation of avidin monomers results in a purification resin with a much lower biotin-binding affinity than native tetrameric avidin, enabling recovery of biotinylated molecules using mild elution conditions.
- Non-denaturing purifies biotinylated products using mild elution conditions (2 mM free biotin)
- Reusable at least 10 times, with marginal loss in biotin binding capacity (approximately 2,5% decrease per regeneration)
- Retains biotin-binding specificity and low non specific binding of native avidin
- Binding capacity is >1,2 mg biotinylated BSA/ml resin