Pierce™ CDI-activated agarose is carbonyldiimidazole affinity chromatography resin, activated for covalent immobilisation of N-nucleophiles and primary amine ligands in aqueous or organic solvent conditions.
- No secondary reagents needed since rection proceeds spontaneously
- Half-life of hydrolysis is much longer than hydroxysuccinimide ester activations
- Reaction is most efficient with primary amines at pH 9 to 11
- Immobilisation occurs through the reaction of N-nucleophiles with 1,1' -carbonyldiimidazole groups of the resin to form a stable, uncharged N-alkylcarbamate linkages
- Available in convenient form as it is supplied as stabilised, 50% slurry in acetone
CDI-activated resin is porous, beaded agarose chromatography resin that is activated with carbonyl diimidazole groups for covalent immobilisation of proteins and other molecules that contain N-nucleophiles. Coupling can be performed in aqueous or organic solvent conditions, making possible the immobilisation of many kinds of ligands. Once a molecule is immobilised, the prepared affinity resin can be used multiple times in typical affinity purification procedures.