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Anti-Phosphotyrosine Mouse Monoclonal Antibody [clone: PY20]

Supplier: MP Biomedicals
A large number of oncogene protein kinases and growth factor receptors will typically phosphorylate tyrosine residues. The PY20 and other phosphotyrosine antibodies have been effective in the characterization of tyrosine kinase substrates involved in oncogenesis, growth regulation and cell differentiation.

  • Working dilution: 1:500 for Immunoprecipitation, Immunohistochemistry, Immunocytochemistry
  • Working dilution: 1:5000 for Western Blot
  • Working dilution: 1:2000 for ELISA

Antibody is packaged in quantities of 100 µg at a concentration of 1 mg/ml in 20 mM sodium phosphate, 150 mM sodium chloride, pH 7,5. It is a highly purified immunoglobulin fraction.

It reacts specifically and strongly to phosphotyrosine containing proteins. It will not recognize phosphothreonine or phosphoserine containing proteins. This antibody is not species specific. It is known to be inhibited by divalent cations (>1 mM) and high salt concentrations (>0,2 M).

Type: Primary
Antigen: Phosphotyrosine
Clonality: Monoclonal
Clone: PY20
Host: Mouse
Isotype: IgG2b



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Size Pk VWR Catalog Number Quantity
100 μg 100 µG ICNA08691371
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Antigen name Phosphotyrosin
Clonality Monoclonal
Antibody type Primary
Clone PY20
Host Mouse
Isotype IgG2b
Western blot Yes
ImmunoChemistry Yes
ImmunoPrecipitation Yes
Concentration 1,0 mg/ml
Immunogen Phosphotyrosine conjugated to carrier protein (PY-KLH)
Storage temperature 0 to +5 °C
Tested applications Immunoblot
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