High binding capacity beads for affinity purification of GST tagged proteins.
- Excellent binding capacity
- High flow rate
- No significant loss of the glutathione ligand
- pH stability range of 2 to 10
Glutathione-Sepharose is prepared by covalently coupling glutathione to epoxy-activated 4% cross-linked sepharose beads to form a stable thioether linkage. The coupling was optimised to give a high binding capacity and could be greater than 10 mg of Glutathione-S-transferase (GST) per ml of wet gel.
Delivery information: Supplied as a 50% slurry in 20% ethanol/ H₂O; >3 mg (10 µmol) glutathione per ml Sepharose beads.