Thrombin-sepharose beads

Supplier: BioVision
BIOV7925-25EA 1800 EUR
BIOV7925-25 BIOV7925-5 BIOV7925-1
Thrombin-sepharose beads
Protein Purification Protein Purification Reagents
Thrombin-Sepharose beads are designed for efficient cleavage of recombinant fusion proteins containing thrombin-specific cleavage sites.

  • Forumlated as 6% cross linked Sepharose beads provided as 50% slurry in pure glycerol
  • Ligand density of 1 mg/ml of the resin
  • It can be regenerated for repeat use

Containing a thrombin cleavage site (e.g. Leu-Val-Pro-Arg-ll-Gly-Ser; ll: cleavage site), circumventing the need for chromatographic techniques to remove thrombin after cleavage reaction is completed. Thrombin Sepharose is prepared by covalent coupling of bovine thrombin to activated 6% cross-linked Sepharose beads. 15 µl of the slurry is sufficient to cleave more than 90% of 1 mg of BioVision’s 6X His-tagged fusion protein in 50 mM Tris buffer, 0,1 M NaCl, pH 8,0 within 4 hours at room temperature.
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