Escherichia coli MDH, also known as Malate dehydrogenase, is a group of multimeric enzymes consisting of identical subunits usually organised as either dimer or tetramers with subunit molecular weights of 30-35 kDa. Its major function is to catalyse the NAD / NADH-dependent interconversion of the substrates malate and oxaloacetate. This reaction plays a key part in the malate / aspartate shuttle across the mitochondrial membrane, and in the tricarboxylic acid cycle within the mitochondrial matrix.MDH is synthesised in several organisms including archaea, eubacteria, fungi, plant and mammals. The enzymes share a common catalytic mechanism and their kinetic properties are similar, which demonstrates a high degree of structural similarity. The three-dimensional structures and elements essential for catalysis are conserved between mitochondrial and cytoplasmic forms of MDH in eukaryotic cells even though these isoenzymes are only marginally related at the level of primary structure.
Fusion-Tag: N-6 His tag
This recombinant protein can be used for biological assays. For research use only.