Pyruvate Kinase Assay Kit provides a simple, direct and automation-ready procedure for measuring pyruvate kinase activity in various biological samples such as blood, tissues, and culture cells, etc. In the assay, PEP and ADP were catalyzed by PK to generate pyruvate and ATP.
- Fast and convenient
- Detects 0,1 mU/ml pyruvate kinase
- Species reactivity: Mammalian
Pyruvate kinase (PK, EC 2.7.1.40) is an enzyme involved in glycolysis. It catalyses the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Lack of pyruvate kinase will slow down the process of glycolysis which causes the disease known as pyruvate kinase deficiency. The generated pyruvate is oxidised by pyruvate oxidase to produce color (at λ = 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Storage at −20 °C.
Delivery information: Kit includes PK assay buffer, OxiRed™ probe (in DMSO), PK enzyme mix, PK substrate mix, PK positive control (~18 mU) and pyruvate standard (100 nmol/µl).