Dot blotting is a simple technique to identify a known protein in a biological sample. The ease and simplicity of the technique makes dot blotting an ideal diagnostic tool.
- Simulated test samples
- Teaches immunodetection technique
- Hands-on Dot Blot Analysis technique with necessary reagents and supplies
The method involves applying small volumes of antigens or test samples on protein binding membrane; the membrane captures and immobilizes proteins. The proteins immobilized on the membrane are probed with a specific antibody and a matching specific detection reagent. In this lab activity students screen test samples from a population and identify those who are carriers of a disease. Students learn how dot blotting applied in the real world, for example in clinical testing, forensic, and research laboratories.
Delivery information: Supplied with components needed for hands-on experimentation for six workstations of 4-5 students or 24-30 students. Supplied with teacher’s guide and separate student’s guides.