6-Carboxy-X-rhodamine succinimidyl ester (6-ROX SE) amine-reactive fluorescent dyeSupplier: Biotium
6-ROX N-succinimidyl ester
, Carboxy-X-rhodamine succinimidyl ester, 6-isomer
, 6-ROX, SE
, 6-Carboxy-X-rhodamine, NHS ester
6-ROX, SE (6-Carboxy-X-rhodamine, succinimidyl ester, single isomer) is the amine-reactive form of 6-carboxy-X-rhodamine single isomer.
- Dark purple solid soluble in DMSO or DMF
- Absorption/Emission 576nm/601nm (MeOH)
- Extinction Coefficient 80,000
- Store desiccated at -20 °C. Protect from light, especially in solution
6-ROX is widely used in DNA sequencing and DNA probes based on fluorescent energy transfer (FRET). The succinimidyl ester reacts readily with primary or secondary amines under mild conditions. A stock solution of the dye can be made in anhydrous DMF or DMSO. The coupling reaction is usually carried out in a pH ~8.5 buffer (for example, 0.1-0.2 M sodium bicarbonate buffer). Alternatively, the coupling reaction may also be carried out in an organic solvent for amino compounds that are insoluble in aqueous solution.
Storage Temperature: Freezer