Safranine O solution for Gram staining, Sigma-Aldrich®

Supplier: Merck
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Synonyms: Safranin TSafranin Y3,7-Diamino-2,8-dimethyl-5-phenylphenazinium chlorideSafranin OGossypimine3,7-Diamino-2,8-dimethyl-5-phenylphenazin-5-ium chlorideCI 50240Basic Red 2Cotton RedSafranin ASafranin

M-Clarity™ quality level = MQ300

1.09217.0500EA 40.3 EUR
1.09217.0500 1.09217.9180 1.09217.2500
Safranine O solution for Gram staining, Sigma-Aldrich®
Safranine O

Counterstain for Gram staining


The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution (Lugol’s solution), a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by Gram's safranin solution and appear orange. A bottle with 500 ml Gram's safranin solution is sufficient for staining up to 250 slides, it is also available as part of the Gram-color stain set. It is an IVD product and CE registered.

Formula: C₂₀H₁₉ClN₄
MW: 350,85 g/mol
Density: 0,98 g/cm3 (20 °C)
Flash Pt: 49 °C
Storage Temperature: Ambient
MDL Number: MFCD00011759
CAS Number: 477-73-6
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Specification Test Results

Suitability for microscopy passes test
gram-positve microorganisms blue-violet
gram-negative microorganisms pink to red

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