Phloxin B, Certistain® for microscopy (C.I. 45410), Sigma-Aldrich®

Supplier: Merck

Synonyms: CyanosinePhloxineAcid Red 92Eosine Blue2′,4′,5′,7′-Tetrabromo-4,5,6,7-tetrachlorofluoresceinEosin S extra bluishCI 45410

1.15926.0025EA 137 EUR
1.15926.0025
Phloxin B, Certistain® for microscopy (C.I. 45410), Sigma-Aldrich®
Phloxin B

The Certistain® dry dye used for cytoplasmic staining in H&E


The CERTISTAIN® dry dye Phloxin B (C.I. 45410) is used for the preparation of a staining solution for cytoplasmic staining in histological sections of human origin. It is mainly used in Hemytoxylin and Eosin (H&E) overview staining that is routinely performed in histological laboratories. Eosin and Phloxine bind to the positively charged plasma proteins. The combination of both dyes results in a broader color range in the tissue.
As CERTISTAIN® dye it is chemically quality-certified according to highest standards and strict specifications. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations.

Formula: C₂₀H₂Br₄Cl₄Na₂O₅
Storage Temperature: Ambient
MDL Number: MFCD00070627
EINECS: 242-355-6
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Specification Test Results

Dye content (gravimetrically) ≥ 80 %
Identity (UV/VIS-Spectrum) passes test
Absorption maximum λ max (ethanol 50 %) 546 - 550 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.005 g/l; ethanol 50 %) 930 - 1400
TLC-Test passes test
Loss on drying (110 °C) ≤ 10 %
Suitability for microscopy passes test

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