Phloxin B, Certistain® for microscopy (C.I. 45410), Sigma-Aldrich®Supplier: Merck
, Acid Red 92
, Eosine Blue
, Eosin S extra bluish
, CI 45410
The Certistain® dry dye used for cytoplasmic staining in H&E
The CERTISTAIN® dry dye Phloxin B (C.I. 45410) is used for the preparation of a staining solution for cytoplasmic staining in histological sections of human origin. It is mainly used in Hemytoxylin and Eosin (H&E) overview staining that is routinely performed in histological laboratories. Eosin and Phloxine bind to the positively charged plasma proteins. The combination of both dyes results in a broader color range in the tissue.
As CERTISTAIN® dye it is chemically quality-certified according to highest standards and strict specifications. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations.
Storage Temperature: Ambient
Specification Test Results
|Dye content (gravimetrically)||≥ 80 %|
|Identity (UV/VIS-Spectrum)||passes test|
|Absorption maximum λ max (ethanol 50 %)||546 - 550 nm|
|Spec. Absorptivity A 1%/1cm (λmax; 0.005 g/l; ethanol 50 %)||930 - 1400|
|Loss on drying (110 °C)||≤ 10 %|
|Suitability for microscopy||passes test|