TISAB, in general, is mixed with samples, as it helps in maintaining the ionic strength and pH constant. It is mainly used to decomplex metal-F complexes.
TISAB II can be prepared using NaCl with glacial acetic acid, 1,2-Cyclohexylenedinitrilotetraacetic acid and NaOH. It also has high concentrations of cation chelators that hydrolyse fluoride-ion pairs to form fluoride ions.
TISAB II has been used to determine total fluoride concentration using fluoride ion sensitive electrode from dental varnish.