3,3',5,5'-Tetramethylbenzidine (TMB) Liquid Chromogenic Substrate

Supplier: Apollo Scientific

Synonyms: TMB

3,3',5,5'-Tetramethylbenzidine (TMB) Liquid Chromogenic Substrate
3,3',5,5'-Tetramethylbenzidine (TMB)

TMB Ready-To-Use chromogenic substrate contains 3,3',5,5'- tetramethylbenzidine and H202 in a Citric Acid buffer and is recommended for use in ELISA procedures. TMB is a substrate for horseradish peroxidase. It produces a soluble blue color product that can be read spectrophotometrically at 370 or 620-650 nm. The reaction may be amplified threefold when stopp ed with acid, generating a yellow color with its absorption maximum at 450nm. After stopping, it should be read within 30 minutes. Not recommended for use in immunohistochemical or blotting assays. Stability: Minimum of 1 year if stored at 2-8 °C.

Formula: C₁₆H₂₀N₂
MW: 240,35 g/mol
Storage Temperature: Refrigerator
MDL Number: MFCD00007748
CAS Number: 54827-17-7

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