Enzymatic assay to measure phosphoenolpyruvate carboxylase activity in biological samples.
- Simple, highly sensitive, high-throughput compatible
Phosphoenolpyruvate carboxylase (EC 4.1.1.31, PPC) is an enzyme found in plants and some types of bacteria. It catalyses the formation of oxaloacetate from phosphoenolpyruvate by fixing one carbon in the forms of either carbon dioxide or bicarbonate. PPC participates in carbon fixation in C4 photosynthesis and Crassulacean acid metabolism (CAM) plants and acts as an anaplerotic enzyme, which replenishes TCA cycle intermediates, in bacteria and C3 plants.
The participation of PPC in the carbon fixation cycle of C4 plants enable them to survive in high sunlight but dry environments. This kit provides a quick and easy method for the measurement of PPC activity in various samples. In this assay, Phosphoenolpyruvate Carboxylase is coupled with a set of enzymes that convert PEP and carbonate into a series of intermediates and hydrogen peroxide, which in turn, reacts with a probe and converter generating a colorimetric signal (OD 570 nm).
The generated absorbance signal is directly proportional to the amount of active PPC present in the samples. This kit provides a simple, fast and high throughput adaptable assay to measure the activities of PPC as low as 0,2 mU.
