Experimentation kits, blue/white cloning of a DNA fragment and assay of ß-galactosidase

Supplier: EDVOTEK EUROPE LTD
EDVO300EA 265 EUR
EDVO300
Experimentation kits, blue/white cloning of a DNA fragment and assay of ß-galactosidase
Educational Materials Biology Educational Materials Biochemistry Learning Activities
When DNA is subcloned in the pUC polylinker region, β-galactosidase production is interrupted, resulting in the inability of cells to hydrolyse X-Gal. This results in the production of white colonies amongst a background of blue colonies. This experiment provides a DNA fragment together with a linear plasmid and T4 DNA ligase. Following the ligation to synthesis the recombinant plasmid, competent E. coli cells are transformed and the number of recombinant antibiotic-resistant white and blue colonies are counted. β-galactosidase activity is assayed from blue and white bacterial cells.

  • For 5 lab groups
  • Ligation 60 minutes; transformation 40 minutes; incubation overnight

Also required: Incubator, water bathes, automatic micropipette and tips, spectrophotometer, centrifuge and microcentrifuge.

Delivery information: Kit includes instructions, linearised pUC plasmid and DNA fragment, T4 ligase, bacterial LyphoCells for transformation, reconstitution buffer, X-Gal in solvent, IPTG, calcium chloride, antibiotic, ReadyPour LB agar, LB media for recovery, growth media, assay components, and plastic supplies.
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