Four experimental modules are combined into one experiment to provide a comprehensive biotechnology exploration focusing on the green fluorescent protein (GFP). Bacterial cells are transformed to express the GFP. The transformed cells are then grown and the GFP purified by column chromatography. Finally, the purity of the protein fractions are analysed by SDS polyacrylamide electrophoresis.
- For 6 lab groups
- Transformation 50 minutes; overnight incubation; chromatography 45 minutes; optional electrophoresis 90 minutes
Also required: Incubator, water baths, automatic micropipette and tips, long wave UV light, ring stand and clamps, lab glassware, ice, vertical gel electrophoresis apparatus and power supply, 3 polyacrylamide gels (12%), glacial acetic acid, and methanol.
Delivery information: Kit includes instructions, transformation cells, plasmid DNA for GFP, IPTG, ampicillin antibiotic, calcium chloride, ReadyPour LB agar, LB media for recovery, petri dishes, pipettes calibrated transfer pipettes, inoculating loops, microtest tubes with attached caps, toothpicks, dry matrix for columns, chromatography columns, green and blue fluorescent protein extracts, elution buffer, protein molecular weight standards, protein denaturation solution, glycerol solution, Tris-Glycine-SDS buffer, and stains.