Kits: in search of the sickle cell gene by southern blot, Ready-to-Load™

Supplier: Edvotek
EDVO315EA 229 EUR
EDVO315
Kits: in search of the sickle cell gene by southern blot, Ready-to-Load™
Educational Materials Biology Educational Materials Genetics Learning Activities
Southern blotting is an important technique used widely in clinical genetics and research. By transferring DNA from an agarose gel onto a membrane the method allows the precise analysis and identification of DNA bands on a gel. Using this kit, students use Southern blotting to find a point mutation in the haemoglobin gene indicating Sickle Cell Anaemia.

  • For five lab groups
  • Electrophoresis, 45 minutes; blotting, overnight; staining and destaining, 10 minutes

Also required: Electrophoresis tank, power supply, water bath and 80 °C incubator.

Delivery information: Kit includes instructions, Ready-to-Load™ DNA samples, agarose, electrophoresis buffer, nylon membranes, filter paper, and blotting stain.
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