Experimentation kits, human PCR Tool Box™

Experimentation kits, human PCR Tool Box™
Educational Materials Biology Educational Materials PCR Learning Activities
Polymerase Chain Reaction (PCR) is commonly used to determine paternity as it is a very sensitive method for DNA analysis. In this experiment, students gain an understanding of the principles behind this non-forensic use of DNA fingerprinting using their own DNA. The kit provides three sets of primers to carry out the PCR amplification of Alu element (PV92) on chromosome 16, the VNTR locus (D1S80) on chromosome 1, and two regions of the mitochondrial gene. Sufficient for 6 runs of each PCR reaction.

  • For 6 lab groups
  • Set up 30 minutes; PCR 2 hours or overnight; electrophoresis 45 minutes

Also required: Micropipettes (5 to 50 µl) with tips, water bath, thermal cycler, electrophoresis tank and power supply.

Delivery information: Kit includes instructions, proteinase K, PCR beads, control and primer DNA, microtubes, chelating agent, agarose, DNA ladder, practice gel loading solution, gel loading dye, electrophoresis buffer, and gel stain.
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