Following restriction enzyme digestion of DNA molecules, researchers need to rejoin the ends of the DNA to generate recombinant DNA, a process known as ligation. Ligation of DNA is achieved with the bacterial enzyme T4 DNA ligase, which catalyses the formation of phosphodiester bonds. The DNA Ligation kit teaches students about ligation as they ligate several DNA fragments together to make larger pieces of DNA that are easily identified by agarose electrophoresis.
- Teaches joining of DNA ends by a process known as ligation
Also required: Agarose electrophoresis equipment.
Delivery information: Supplied with components needed for hands-on experimentation for six workstations of 4 to 5 students or 24 to 30 students. Supplied with Teacher’s Guide and separate Student’s Guides.