A hands on lab activity to study immunoaffinity chromatography and use a specific antibody to purify antigenic proteins from complex samples. This technique involves performing a chromatography procedure using antigen or antibody immobilised on a chromatographic resin. The solution containing antigen or antibody is passed through the column, which specifically and efficiently captures antibodies (antigen). The captured molecules are eluted from the column as a pure fraction. In this lab activity, students perform immunoaffinity chromatography and learn how this method is utilised in research laboratories.
- Teaches antibody:antigen precipitation
- Hands-on activity to precipitate antigen and antibody complex
- Quantitative assessment of antibody concentration
Also required: Low speed centrifuge for 1,5 to 2 ml tubes, rotator to hold 1,5 ml tubes or shaking incubator, UV light box.
Delivery information: Supplied with components needed for hands-on experimentation for six workstations of 4 to 5 students or 24 to 30 students. Supplied with Teacher’s Guide and separate Student’s Guides.