The 2-D Clean-Up Kit is designed to prepare samples for 2-D electrophoresis that otherwise produce poor 2-D spot-maps due to high conductivity, high levels of interfering substances, or low protein concentration. The reagents quantitatively precipitate proteins while leaving interfering substances, such as detergents, salts, lipids, phenolics, and nucleic acids, in solution.
- Improves the quality of 2-D electrophoresis results by removing interfering contaminants
- Quantitative precipitation separates proteins from detergents, salts, lipids, phenolics, and nucleic acids
- Delivers quantitative yield in about 90 minutes
Treatment of the sample with 2-D Clean-Up Kit can improve the quality of 2-D electrophoresis results, reducing streaking, background staining, and other artifacts due to interfering contaminants. The kit can make 2-D analysis possible for samples that are otherwise too dirty or too dilute.
The 2-D Clean-Up Kit procedure uses precipitant and co-precipitant in combination to quantitatively precipitate proteins. Precipitated proteins are pelleted by centrifugation and the precipitate is further washed to remove nonprotein contaminants. After a second centrifugation, the resultant pellet is resuspended into denaturing sample solution for first-dimension IEF.
The procedure can be completed in 90 minutes and does not result in spot gain or loss. The kit contains sufficient reagents to process 50 samples of up to 100 μl each. The procedure can be scaled up for larger volumes or more dilute samples.
2-D Clean-Up Kit increases labeling efficiency and protein abundance when using 2-D Fluorescence Difference Gel Electrophoresis (2-D DIGE).
Ordering information: Kit contains 15 ml Precipitant, 17,5 ml Coprecipitant, 50 ml Wash buffer, 250 μl Wash additive, Quick reference card, and instructions.