You Searched For: Nucleic Acid Purification Kits and Reagents

Supplier: OMEGA BIO-TEK

Description: The E.Z.N.A.® Plant DNA DS Mini Kit is designed for efficient recovery of genomic DNA up to 30 kb in size from fresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols or having a lower DNA content. Up to 50 mg wet tissue can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind® matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridisation applications.

MSDS

Supplier: VWR Chemicals

Description: Purifies DNA bands 70 bp to 20 kb in length from agarose gels. The DNA band of interest is excised from the gel, dissolved in binding buffer, and efficiently processed in a peqGOLD DNA column. >90% recovery. Elution volume: 30 to 50 µl.

MSDS

Catalog Number: (27-5130-01)

Supplier: Cytiva

Description: illustra™ MicroSpin™ S-300 HR columns designed for rapid purification of PCR products (>100 bp) from unincorporated primers (<20-mers) and nucleotides using spin-column chromatography.

UOM: 1 * 50 items

Sale

Supplier: OMEGA BIO-TEK

Description: The E.Z.N.A.® blood DNA kit provides rapid total DNA isolation from up to 250 µl of fresh or frozen anticoagulated whole blood. The E.Z.N.A.® blood DNA kit can also be used for the preparation of genomic DNA from buffy coat, serum, plasma, bone marrow, lymphocytes, platelets, and body fluids. This kit allows for simultaneous processing of single or multiple samples in less than 30 minutes. Phenol/chloroform extractions, and time-consuming steps such as precipitation with isopropanol or ethanol have been eliminated. DNA purified with the E.Z.N.A.® blood DNA method is ready for applications such as PCR, southern blotting, or restriction enzyme digestion.

MSDS

Supplier: OMEGA BIO-TEK

Description: Isolate total RNA from cells or soft tissues using spin column.

MSDS Certificates

Supplier: OMEGA BIO-TEK

Description: The E.Z.N.A.® tissue DNA kit offers a simple, rapid, and cost effective method for the isolation of DNA from a wide variety of sample sources including fresh or frozen animal cells and tissues. After cell lysis, the DNA purification process can be completed in less than 30 minutes. Up to 30 mg of tissue at a time can be readily processed at a time using the simple E.Z.N.A.® tissue DNA protocol. Single or multiple samples can be simultaneously processed with this spin-column based kit. There is no need for phenol/chloroform extractions, or time-consuming steps such as precipitation with isopropanol or ethanol. DNA purified using the E.Z.N.A.® tissue DNA kit is ready for most downstream applications such as PCR, southern blot and restriction enzyme digestion.

MSDS Certificates

Supplier: OMEGA BIO-TEK

Description: Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins that can significantly interfere with transfection experiments downstream. The E.Z.N.A.® Endo-Free Plasmid kits integrate an efficient endotoxin removal step into the plasmid purification procedure to produce high quality transfection grade plasmid.

MSDS

Supplier: OMEGA BIO-TEK

Description: Gel purification of DNA is a common technique used for the isolation of specific DNA fragments. However, most methods either fail to completely remove agarose (which can lead to problems in downstream manipulations), shear the DNA, or result in very low yields.

MSDS Certificates

Supplier: OMEGA BIO-TEK

Description: RNA-Solv® Reagent is a one reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidine isothiocyanate, is a modification of the single-step RNA isolation method developed by Chomczynski and Sacchi. The sample is homogenised and lysed in RNA-Solv® Reagent, which maintains the integrity of the RNA while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.

MSDS Certificates

Supplier: OMEGA BIO-TEK

Description: The E.Z.N.A.® bacterial DNA kit allows the rapid and reliable isolation of high quality total cellular DNA from a wide variety of bacterial species. This kit uses optimised lysis condition and up to 1×10⁹ bacterial cells can be processed for each column. There are no organic extractions, thus reducing plastic waste and hands on time to allow multiple samples to be processed in parallel. Bacterial cells are grown to log-phase and harvested. The cell wall is removed by lysozyme digestion and bead beating, followed by protease digestion. Following lysis, binding conditions are adjusted and the sample is applied to a HiBind DNA spin-column. Two rapid wash steps remove trace salts and protein contaminants, and DNA is finally eluted in water or elution buffer. Purified DNA can be directly used in downstream applications without the need for further purification.

MSDS

Supplier: VWR Chemicals

Description: Easy to use method for isolation up to 100 µg of total RNA from cultured eukaryotic cells and soft tissues <1×10⁷ cells or 30 mg tissue.

MSDS Sale

Supplier: Quantabio

Description: 5PRIME Phase Lock Gel (PLG) eliminates interphase-protein contamination during phenol extraction and ensures faster results with improved recoveries. PLG migrates to form a tight seal between the phases of an aqueous/organic extraction during centrifugation. Organic phase and interphase materials are effectively trapped in or below the barrier, thus enabling complete and easy decanting or pipetting of an entire aqueous phase. The benefits are increased yields of up to 30%, increased protection from exposure to hazardous compounds, and no risk of interphase sample contamination. For convenience, PLG is provided pre-dispensed into standard centrifuge tubes of various sizes. PLG is available in two different density formulations – Heavy and Light – and can be used in virtually any protocol that calls for extraction with phenol and/or chloroform, simply by selecting the formulation suitable for organic and aqueous phases.

MSDS

Catalog Number: (30-2010)

Supplier: VWR Chemicals

Description: TriFast™ is a complete ready to use reagent for simultaneous isolation of RNA, DNA and proteins from liquid samples, tissue and cells.

UOM: 1 * 100 mL

MSDS Certificates Sale

Supplier: Cytiva

Description: Sephadex G-50 grade F is a size exclusion chromatography resin designed for desalting and buffer exchange, and for purification of DNA and oligonucleotides greater than 20 bases in length using spin columns.

MSDS

Supplier: OMEGA BIO-TEK

Description: E.Z.N.A.® Plasmid Midi Kit offers a novel and reliable method of isolating up to 200 µg of plasmid DNA in less than 60 minutes. This kit combines HiBind® membrane technology with the time-tested consistency of alkaline SDS lysis of bacterial cells to deliver high-quality plasmid DNA. The DNase/RNase-free HiBind® DNA midi columns operate by eliminating time consuming phenol-chloroform extractions and alcohol precipitations. Plasmid purification using the E.Z.N.A.® Plasmid Midi Kit follows a simple bind-wash-elute procedure thereby making it possible for multiple samples to be processed. Although yields vary according to plasmid copy number, <i>E.coli </i>strain, and conditions of growth, 50 ml of an overnight culture in LB medium will typically produce 200 µg of high-copy number plasmid DNA. When working with low-copy number plasmids the starting culture volume can be increased up to 100 ml. High-quality DNA is ready for immediate use in routine molecular biology applications, such as automated fluorescent sequencing, restriction enzyme digestion, and transfection screening.

MSDS

Catalog Number: (27-5140-01)

Supplier: Cytiva

Description: illustra™ MicroSpin™ S-400 HR columns designed for rapid purification of PCR products (>200 bp) from unincorporated primers (<32-mers) and nucleotides using spin-column chromatography.

UOM: 1 * 50 items

Sale