You Searched For: Protein Purification Reagents

Protein purification reagents are for use in isolating one or more proteins from a complex mixture, usually cells, tissues, or whole organisms. This process is vital for function and structure characterization, as well as identifying interactions of the protein of interest. Important during the many steps, the protein purification reagents assist with cell lysis, separating the soluble protein components from cell debris, and finally separating the protein of interest from product- and process-related impurities.

Protein purification reagents are for use in isolating one or more proteins from a complex mixture, usually cells, tissues, or whole organisms. This process is vital for function and structure characterization, as well as identifying interactions of the protein of interest. Important during the many steps, the protein purification reagents assist with cell lysis, separating the soluble protein components from cell debris, and finally separating the protein of interest from product- and process-related impurities.


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Supplier: Avantor
Description: J.T.Baker® BAKERBOND® supported liquid extraction (SLE) plates are an ideal and faster alternative to traditional liquid-liquid extraction methods and allow excellent recoveries of analytes from biological matrices.

Supplier: Avantor
Description: J.T.Baker® BAKERBOND® protein precipitation plates enable fast protein removal from biological samples.

Supplier: G-Biosciences
Description: Jacalin, or <i>Artocarpus integrifolia</i> lectin, is a tetrameric two-chain lectin with a molecular weight of 66 kDa. It is a α-D-galactose binding lectin purified from jack fruit (<i>Artocarpus integrifolia</i>) seeds.

Supplier: G-Biosciences
Description: Concanavalin A (Con A) agarose consists of Con A coupled to 6% agarose by the cyanogen bromide method. Con A is a tetrameric metalloprotein lectin isolated from <i>Canavalia ensiformis</i> (jack bean). It is used for the purification of glycoproteins, polysaccharides and glycolipids as it binds molecules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues. It has also be used in other application areas including purification of enzyme-antibody conjugates, purification of IgM and separation of membrane vesicles. As stated above, it is a metalloprotein and to maintain its binding characteristics the presence of both Mn²⁺ and Ca²⁺ is essential. Each subunit of Con A utilises one calcium and one manganese ion and these cations can be removed under acidic conditions abolishing the carbohydrate-binding activity.

Catalog Number: (786-243)
Supplier: G-Biosciences
Description: Following digestion of proteins with proteases, the addition of Pep-Extract™ rapidly elutes digested peptides in under 20 minutes. The eluted peptides can be used directly in mass spectrometry analysis without any additional clean up. Contains sufficient for 500 protein spots.
UOM: 1 * 100 Tests


Catalog Number: (786-391)
Supplier: G-Biosciences
Description: Immobilised D-Galactose is designed for the rapid purification of lectins, galactosidases and other galactose-binding molecules. The resin is ideal for the purification of agglutinins, lectins, toxins, glactose-binding, N-acetylgalactosaminebinding or carbohydrate binding molecules. Specific applications include the purification of galactosidases, C‐type lectins, enterotoxins and cholera toxin.
UOM: 1 * 5 mL


Supplier: G-Biosciences
Description: Protein preparations specifically prepared for standardising electrophoresis methods and protocols. Substantially free from non-protein agents such as nucleic acids, detergents, salts, lipids and other common laboratory agents, the protein preparation has low conductivity (\<50 μs). Supplied as dry protein pellets, simply re-hydrate and use. Proteomic protein sample controls are available for animal cells, <i>E.coli</i>, yeast, plant or as a control set that contains one 2 mg vial of each.

Supplier: Thermo Fisher Scientific
Description: The HeLa Protein Digest Standard is a highly validated mammalian protein digest that may be used as a quality control sample for mass spectrometry (MS) analysis of complex proteomic samples.

Catalog Number: (PIER77720)
Supplier: Thermo Fisher Scientific
Description: Bond-Breaker™ TCEP Solution (neutral pH) is a stable, 0,5 M solution of the thiol-free, phosphine-based TCEP compound, useful as a 10X stock for addition to SDS-PAGE sample loading buffers to reduce protein disulphide bonds.
UOM: 1 * 5 mL

MSDS


Catalog Number: (PIER90034)
Supplier: Thermo Fisher Scientific
Description: Pierce™ Iodoacetamide is a sulphhydryl-reactive alkylating reagent used to block reduced cysteine residues for protein characterisation and peptide mapping.
UOM: 1 * 1 SET


Supplier: Thermo Fisher Scientific
Description: Pierce™ Recombinant Protein A is useful as the basis for preparing various kinds of probes or affinity media for detection or purification of rabbit and human antibodies, especially IgG isotypes, in immunoassays and antibody purification protocols.

Supplier: Thermo Fisher Scientific
Description: Pierce™ MM(PEG)<sub>n</sub> reagents are methyl-terminated PEG compounds activated with a maleimide group for covalent pegylation of sulfhydryls on proteins or assay surfaces.

Catalog Number: (PIER32400)
Supplier: Thermo Fisher Scientific
Description: Pierce™ Recombinant Peroxidase Conjugated Protein A can be used to probe and detect rabbit and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.
UOM: 1 * 1 mg

MSDS


Catalog Number: (PIER32420)
Supplier: Thermo Fisher Scientific
Description: Pierce™ Recombinant Peroxidase Conjugated Protein L can be used to probe and detect rabbit and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.
UOM: 1 * 0,5 mg


Supplier: Thermo Fisher Scientific
Description: Purified (unconjugated) Pierce™ Recombinant Protein A/G is useful as the basis for preparing various kinds of probes or affinity media for detection or purification of rabbit and human antibodies, especially IgG isotypes, in immunoassays and antibody purification protocols.

Catalog Number: (PIER31007)
Supplier: Thermo Fisher Scientific
Description: Thermo Scientific™ Maleimide Activated NeutrAvidin™ Protein is a specially-prepared form of avidin that decreases background in biotin-binding.
UOM: 1 * 5 mg


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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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